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i&i Prague supports the innovative start-up PEP-Therapy in their extension of the Series-A financing, totalling €5.4 million to progress the clinical development of the lead candidate PEP-010 PEP-Therapy, a biotechnology company developing cell penetrating peptides as targeted therapies for the treatment of cancers, announced today that it raised an additional €2.6 million ($3 million) in an extension of its Series A financing round, bringing the total raised in this round to €5.4 million ($6.4 million). This new funding comprises €1.6 million in equity from Anaxago, i&i Prague and BADGE as well as a €1 million loan from Bpifrance. This increased financial support highlights the potential of PEP-010, as well as PEP-Therapy’s Cell Penetrating & Interfering Peptide (CP&IP) technology platform, which was first developed at Sorbonne University and Institut Curie. PEP-010 is the first of a new class of therapeutic peptides based on PEP-Therapy’s innovative Cell Penetrating & Interfering Peptide (CP&IP) technology. These innovative molecules penetrate cells and specifically block relevant intracellular protein-protein interactions, leading to the inhibition of key pathological mechanisms, without altering physiological mechanisms. PEP-Therapy will use the funds to finance the Phase I a/b clinical trial of PEP-010, PEP-Therapy’s lead candidate, for the treatment of advanced solid tumors. The first part of the Series A, which closed in April 2021, will finance the Phase Ia dose escalation part of the study, with the additional funds being used for the development of PEP-010 until the end of the expansion cohorts, Phase Ib. PEP-Therapy expects to generate promising clinical data from this study, particularly in two indications: metastatic triple negative breast cancer and platinum resistant ovarian cancer. Patients with these two types of solid tumors have a poor prognosis and limited therapeutic alternatives. Antoine Prestat, CEO and co-founder of PEP-Therapy, said: “We are delighted to have completed this financing round via an attractive balance of dilutive and non-dilutive funds from new high quality and diversified investors who will bring expertise and new insights to support our development.” Jaromír Zahrádka, PhD, CEO of i&i, commented: “PEP-Therapy has developed an extensive knowledge of targeted peptides and the promising preclinical data the company has generated show great potential. We are looking forward to seeing the confirmation of the positive results seen in preclinical data in the upcoming Phase I study.” Gaston Vasseur, Investment Manager at Anaxago, added “This extended financing highlights PEPTherapy’s capacity to attract highly specialized as well as diversified investors. The Company has managed to rapidly secure the funding for the Phase I trial with PEP-010, a very important milestone for the company. We are happy to contribute to this financing round in conjunction with a number of experienced life science investors.” In addition, PEP-Therapy and its clinical partners, Institut Curie and Gustave Roussy, previously received a €2.9 million grant from the French state innovation fund – Fonds Unique Interministériel (FUI) – to finance nonclinical and early clinical development of PEP-010. About Anaxago Founded in 2012, Anaxago Venture Capital is a digital VC firm specialised in Healthtech & Biotech, Proptech, Fintech and Impact investing. Anaxago Venture Capital has financed more than 45 companies since its creation. About BADGE Business Angels des Grandes Ecoles (BADGE), created in 2004, is a leading French Business Angels association with more than 280 members, and having participated in 21 fundraisings in 2020 of a combined value of over €5.3 million. Its members are recognized leaders in all sectors of the industry and are willing to finance and support young innovative companies with high growth potential. BADG aims to: Identify and review promising innovative projects Conduct fund-raising for the most promising ones Support the entrepreneurs with individualized business advice and key contacts Provide training and methodological support to its members BADGE has thus financed more than 185 young companies since its creation.

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From the Labs of Roche and Pfizer Back to the Czech Republic: Jan Berka Helps Biotech Startups Grow Globally After spending three decades in the United States—working at companies like Roche, Pfizer, and Adaptive Biotechnologies—Jan Berka has decided to return to the Czech Republic part-time. Today, he serves as Project Manager for International Contacts at i&i Prague. We spoke with him about his journey, professional insights, and why it's important to occasionally look up from the details and focus on the big picture. You’ve had an impressive career in the US. What brought you back to the Czech Republic and led you to join i&i Prague? It was a bit of an impulsive decision. After 30 years in the US, I became genuinely curious about what was happening in biotech back in the Czech Republic. While I visited frequently for holidays, I had lost touch with the local professional scene. At the same time, my wife and I began to miss more regular contact with the Czech landscape, culture, and our friends here. So, when the opportunity to join i&i Prague came up, I didn’t hesitate for a second. You’ve worked for global companies like Roche, Pfizer, and Adaptive Biotechnologies. How has that environment influenced your work at i&i Prague? Above all, it gave me experience. Every work environment I’ve been part of was unique in some way. That gave me valuable insight into what works under different circumstances—and what doesn’t. I now aim to bring that know-how to i&i Prague. Each of our startups is different, and I believe my experience can help support their growth. But we're still at the beginning—I'm curious myself to see how I’ll be able to contribute. As a Project Manager focused on international connections, what do you primarily concentrate on? My role has two main aspects. First, there’s classic project management—guiding the individual projects that go through our incubation program. Second, and just as important, is leveraging the international network I’ve built over the years. It's not just about having good ideas or solutions—you also need to know who to call. That’s my strength. Thanks to longstanding personal connections, I not only know what’s being worked on around the world, but I also know the people behind it. And personal relationships are always the most valuable. I think we often build our own mental ceilings and fail to see beyond them—and that can prevent us from aiming higher. JAN BERKA Project Manager for International Contacts at i&i Prague What has been the most pivotal project or moment in your career so far? Two moments come to mind. The first was my time with the startup 454 Life Sciences. We began as a team of four people with an idea almost no one believed in. But within five years, we launched a genome sequencer that kicked off the entire field of Next Generation Sequencing (NGS). That was a truly unique moment—driven by healthy ambition, vision, and the right team. The second breakthrough happened at Pfizer. We were trying to apply genetics to personalized medicine, but it wasn’t yielding great results. Then a colleague suggested we shift our focus to the immunome, the most variable part of the human genome. That opened up a completely new field of research for us. It was a moment that taught me the importance of seeing things from a different perspective. Ultimately, that shift helped us succeed and led to the development of immunome analysis using NGS. Company culture often plays a key role in startups. In your view, what’s the biggest mindset difference between the US and the Czech Republic? More and more, I get the impression that in the Czech Republic, we tend to focus too much on details—technical or personal. We spend hours discussing issues that are not immediately relevant. In the US, the approach is to pursue the vision, the big picture. The details get resolved along the way. It reminds me of a book by Mojmír Hampl called “For Czechs, the Sky Is Too Low.” I think we often build our own mental ceilings and fail to see beyond them—and that can prevent us from aiming higher. You split your time between Prague and Colorado. What do you enjoy about both places, and how do you balance such different worlds? Surprisingly easily—neither my wife nor I can stay in one place for too long. Prague and Colorado are completely different worlds, and we enjoy the best of both. In Prague, we go to the theatre or a great restaurant. Colorado is all about nature, hiking, and the desert. You’re currently learning to fly. What inspired you to take up piloting? I started flying gliders as a student and did it for about 15 years before life pulled me in other directions. Now that I have more time for hobbies again, I decided to return to flying—and fulfill a childhood dream of piloting a plane with an engine. Since I was young, I admired the “big guys” who could just fly wherever they wanted. Meanwhile, we in gliders had to land in a field if the thermals weren’t favorable. So, I didn’t hesitate—I enrolled in ultralight pilot training and I’m currently preparing for the final exam. Flying is highly technical. You need to understand all the instruments and also what’s happening around you. That’s very similar to science, where you have to know what you’re doing and think analytically. JAN BERKA Project Manager for International Contacts at i&i Prague Cycling is another of your passions. Do you see any parallels between your hobbies and your work in science? Absolutely—and very different ones. Flying is highly technical. You need to understand all the instruments and also what’s happening around you. That’s very similar to science, where you have to know what you’re doing and think analytically. In contrast, mountain biking is all about intuition—pure flow. You don’t think; you react. And it’s during those intuitive moments that I often get my best scientific ideas. I usually rush home afterwards so I don’t forget them. What fascinates you most in biotech right now—and why? I’ve spent my whole career working on genome sequencing, hoping it would become a routine diagnostic tool. It hasn’t happened yet, but we’re getting closer. I’m also fascinated—and somewhat alarmed—by CRISPR-Cas genome editing. The first case where a point mutation was corrected in a child’s liver completely blew my mind—but also scared me. I don’t think society is ready for the sheer power of such technologies. It’s not just scientific progress—it’s a social challenge, too. If there was one thing you could do at i&i Prague, what would it be? I’d be happy to be part of a process where an idea turns into a real product or service that truly helps people. I don’t need to be there at the finish line—but if I can help kick something off that has a meaningful impact, I’ll consider it a success. Thank you for the interview. Author: Martin Kovalčík

CasInvent Pharma, a New University Spin-Off Focused on Developing Cancer Drugs Established with Help of i&i Prague! A new spin-off company, CasInvent Pharma, a.s., has been established by Masaryk University (MU) in cooperation with its investment partner, i&i Prague, s.r.o. (Ltd), in order to facilitate further development of new compounds that could be used to treat certain types of leukaemia, lymphoma and solid tumours. The company will test the compounds that inhibit the enzyme Casein Kinase 1 (CK1) which is responsible, among other things, for the migration of leukaemia cells into lymphoid organs. The research groups of Vitězslav Bryja and Kamil Paruch from the Faculty of Science MU have been studying and developing these compounds for a long time now. Thanks to the newly-established company, they will be able to finish the preclinical stage of the development of these prospective drugs and move on to clinical trials of the most promising compounds. “The main reason behind establishing this spin-off company is the fact that it is virtually the only option to commercialize this technology, which is still in early stages of development, and advance the research to such stage that it catches the interest of big investors from the pharmaceutical industry,” says the director of Technology Transfer Office MU, Eva Janouškovcová, as to why the joint-stock spin-off company was established with the university as one of its stakeholders. “The i&i Prague company searchs and supports the most promising technologies and the highest quality projects. We have known for a long time that the inhibitors of CK1 are the most promising Czech projects. I am glad that Masaryk University has chosen the i&i Prague as a strategic partner for this project. I believe that we will be able to make significant progress towards clinical trials in the development of the drug, attract other co-investors and international partners and enable the practical use of these new substances as soon as possible thanks to the establishment of the CasInvent Pharma company,” said the CEO of i&i Prague, Jaromír Zahrádka. The CasInvent Pharma spin-off will continue developing the promising results of the scientific teams which led to the patenting of the inhibitors, i.e. compounds that significantly decrease or completely inhibit the activity of CK1 and can be used to treat e.g. certain types of leukaemia. More specifically, the B-Cell Chronic Lymphocytic Leukaemia (CLL) and the Acute Myeloid Leukaemia (AML). CLL characteristically causes the accumulation of dysfunctional cancer cells in the blood and their migration to lymph nodes, liver, spleen and bone marrow, which causes further complications such as enlargement of organs, immunodeficiency, anaemia and other. The aggressiveness of the disease then depends on the interaction of these dysfunctional cells with their immediate surroundings – so-called microenvironment. This interaction leads to tumour cells dividing uncontrollably. The inhibitors of CK1 can effectively stop the migration of the leukaemia cells to lymphoid organs, which prevents them from being damaged and hinders the spreading of CLL. AML, which is one of the most aggressive and difficult-to-treat types of leukaemia, has CK1 working in a different way. Based on the current results, the scientists expect the new compounds to initiate programmed cell death (so-called apoptosis) in leukaemia cells. Targeting interactions in the microenvironment, regulating apoptosis and inhibiting migration mechanisms can be successfully used with other forms of cancer as well, e.g. solid tumours the growth and spreading of which often depends on roughly the same principles. CasInvent Pharma joined the ranks of the other 17 spin-off companies Masaryk University helped to establish. The purpose of these spin-off companies is mainly to utilize the university’s intellectual property and increase its value. The university grants companies intellectual property licensing agreements and, in some cases, it even owns shares of the company. As a result, the spin-off companies established by the university bring profit to all the parties involved: for the university, it is the best way to appreciate its intellectual property, the spin-off company gains a competitive advantage on the market and, last but not least, the end product reaches the consumer more quickly

David Stíbal: We believe a startup is one of the best paths nowadays to develop interesting technologies beyond the academic level How complex is the path to a new drug? Why do licenses for Czech drugs end up abroad? What are the most common issues currently faced by biotech startups? David Stíbal, Vice Director of the biotech incubator and venture builder i&i Prague, s.r.o., reflects on these and other questions related to the commercialization of scientific discoveries. How complex is the path to a new drug today? The path to a new drug is very complex and risky, and in some respects, this is even more true today than it was in the past. For example, if we were to calculate the current cost of developing a new drug from initial trials to the final "pill", statistics show it would approach one billion dollars. This figure includes also the costs of all unsuccessful candidates within the development program, preclinical and clinical experiments, production, marketing, and much more. Pharmaceutical companies then need to recoup this investment from the sales of the drugs that make it all the way to market. What drugs are in the highest demand today? According to the list of the most successful drugs (known as "blockbusters," i.e., drugs that generate at least one billion USD annually) for 2023, the most sought-after drugs are GLP-1 agonists, such as anti-obesity drugs (Ozempic, Mounjaro, etc.), cancer-fighting antibodies (e.g., Keytruda from MSD), immunologic drugs (Humira, Stelara, etc.), or HIV medication, incidentally the one developed by Professor Holý’s team at the Institute of Organic Chemistry and Biochemistry in the late 20th century (Tenofovir alafenamide, marketed by Gilead Sciences). Many licenses for Czech drugs are sold abroad. Is there any way to change that? Licenses are typically sold at an early development stage to larger companies that have the capacity to bring such programs through advanced clinical trials and to market. In our field, this is usually the domain of major pharmaceutical companies capable of bearing the high costs mentioned earlier. The idea of having a drug, invented and developed in the Czech Republic, also manufactured here is certainly appealing. However, until a major pharmaceutical company transfers its R&D or manufacturing facility to the Czech Republic, or until a mid- to large-sized pharmaceutical company emerges here, this scenario is hard to imagine. You support startups focused on developing new drugs or diagnostic methods. Are there any positive trends in this segment in the Czech Republic? Through our work, we see a positive trend, especially in the growing number of startups and the willingness of scientists and university employees to discuss commercialization and consider developing their inventions beyond scientific publications or patents. We’re delighted by this, as we believe a startup is one of the best paths nowadays to develop interesting technologies beyond the academic level. However, it's also worth noting that the startup mentality in the Czech Republic, particularly in our field, is not yet as developed as it is in more advanced countries. What are the biggest challenges these startups face today? The biggest issue, globally, is the current lack of investment capital and, more specifically, the scarcity of investors willing to support new, high-risk projects. This is especially true when compared to the “COVID years” of 2019-2021, when funds were released more readily, with investors supporting projects that could, among other things, respond immediately to the crisis. The situation is different now: investors are more cautious about where they allocate their funds, partly because they are reserving capital to support their existing portfolio companies. A startup may be a suitable path, where scientists and managers can work together to further develop the technology until it is relevant for direct licensing to a larger firm. DAVID STÍBAL VICE DIRECTOR AT i&i PRAGUE What did you think of this year’s Prague.bio Conference? I consider the event a great success. It’s impressive how the Prague.bio association managed to organize a high-quality conference with such a small team. Similar events are held fairly regularly across Europe, some of which i&i Prague likes to attend. However, there are relatively few conferences focused on such early-stage technologies, as was the case with Prague.bio. We were also pleased with the scientifically oriented program and high attendance, which shows that such an event is needed in the Czech Republic and is appreciated by both academic and industry players. Are there any new figures in today’s biomedicine who might follow in Professor Holý's footsteps? Repeating such an achievement will be challenging but not impossible. To make it happen, however, we need to build a more robust infrastructure that can help scientists navigate the very complex process of bringing their ideas to fruition. Our incubator aims to be one of the pieces that form this mosaic. What is the best way to commercialize the results of science and research? The answer to this question depends on how advanced the result is and how well it aligns with the current market needs. If it is possible to directly connect with a larger company and license the result to them, this is a relatively fast way to transfer the technology into practice. If that’s not possible, a startup may be a suitable path, where scientists and managers can work together to further develop the technology until it is relevant for direct licensing to a larger firm. Alternatively, developing the technology all the way to market can also be considered, although this is more of an exception in our field. David Stíbal is the Managing Director of the biotech incubator i&i Prague. He evaluates the business potential of scientific projects, establishes and initially manages startups, and works on the long-term development of projects all the way to their successful commercialization. He completed his bachelor’s and master’s degrees at the University of Chemistry and Technology in Prague, earned a Ph.D. from the University of Neuchâtel in Switzerland, and conducted postdoctoral research at the prestigious Massachusetts Institute of Technology (MIT). Czech version of the interview was published in Ekonomický magazín . Author: Martin Kovalčík

CasInvent Pharma raises € 1.6 million from i&i Prague and Other Investors to Support the Development of CK1 Inhibitors for Treatment-Resistant Solid and Hematological Tumours The financing will boost the development of innovative casein kinase 1 (CK1) inhibitors with the potential to treat conditions like acute myeloid leukaemia, pancreatic cancer, and certain types of breast cancer. This investment round was supported by the existing and two new investors. The syndicate includes i&i Biotech Fund (i&i Bio), KHAN Technology Transfer Fund I (KHAN-I), i&i Prague, the Holeček Family Foundation, and BIOINVESTIMED a. s. CasInvent Pharma, a university spin-off focused on developing anti-cancer compounds, has successfully closed the Pre-Series A funding round, raising a total of € 1.6 million from both existing and new investors. Founded in 2020 as a spin-off from Masaryk University in Brno and with the support of i&i Prague, CasInvent Pharma specializes in the development of highly selective inhibitors targeting the casein kinase 1 enzyme family. These enzymes play an important role in several disease-related processes, including the migration of leukaemia cells into lymphoid organs. The small-molecule inhibitors are designed to target individual isoforms of CK1 and thereby selectively eradicate leukemic cells. "It's gratifying to have convinced both new and existing partners to support our quest in developing effective treatment options and hope for patients suffering from these cancers with a very low survival rate," says Alexander Scheer, CEO of CasInvent Pharma. He believes these inhibitors have the potential to treat patients with conditions like acute myeloid leukaemia, pancreatic cancer, certain types of breast cancer (TNBC), and other diseases with limited treatment options. "The company's ability to attract new investors reaffirms not just the project's quality, but also the combined expertise of the team at CasInvent Pharma," says Ivan Vohlmuth, a partner at i&i Bio. "The team has worked really hard over the past 18 months and achieved promising data in the development of their CK1 inhibitor portfolio. We are pleased to continue to support the company," add Michael Krebs and Peter Nussbaumer from KHAN-I. Joining the investment round, alongside i&i Bio, KHAN-I and i&i Prague, are two new partners: the Holeček Family Foundation and BIOINVESTIMED a. s. "Supporting this innovative project allows us to participate in the important development of new treatment methods and therapeutic strategies in the field of oncological diseases. At the same time, we are impressed that a Czech entity has embarked on this difficult and financially demanding path. With our engagement we would like to motivate other investors to fund promising life science companies in the Czech Republic," says Ondřej Novák, Director of the Holeček Family Foundation. Contact: Martin Kovalčík, kovalcik@iniprague.com , +420 777 472 863 About i&i Bio i&i Bio is a Luxembourg-based venture capital firm that invests in innovative European life sciences companies focused on drug discovery, medical devices, diagnostics, and digital health. The Fund was created thanks to the cooperation of the biotech incubator i&i Prague and the European Investment Fund (see below). With over € 53 million under management, i&i Bio plans to invest in about 20 early-stage companies. i&i Bio is led by an experienced team of professionals with backgrounds in private equity, healthcare and venture capital supporting entrepreneurs on their journey to global success. Thanks to close cooperation with the fund’s main sponsor, the biotech academic incubator i&i Prague, i&i Bio is supporting and advancing transformative Central European technology companies. For more information visit www.inibio.eu . About CasInvent Pharma CasInvent Pharma is a drug discovery spin-off company established in 2020 by Masaryk University (MU) and Bio-Innovation Centre i&i Prague, s.r.o. (Ltd). The mission of the company is to develop new therapeutic options for the treatment of resistant tumours. The CasInvent platform is based on the use of proprietary, best-in-class highly selective inhibitors of enzymes belonging to the casein kinase 1 (CK1) family that are responsible for the regulation of different cellular mechanisms leading to resistance to targeted therapies. More information at www.casinvent.com . About European Investment Fund i&i Bio is supported by an investment from the EIF, with the support of: lnnovFin Equity, with the financial backing of the European Union under Horizon 2020 Financial Instruments and the European Fund for Strategic Investments (EFSI) set up under the Investment Plan for Europe. The purpose of EFSI is to help support financing and implementing productive investments in the European Union and to ensure increased access to financing; and the Pan-European Guarantee Fund (EGF), implemented by the EIF with the financial support of the Participating Member States. The objective of EGF is to respond to the economic impact of the COVID-19 pandemic by ensuring that companies in the Participating Member States have sufficient short-term liquidity available to weather the crisis and are able to continue their growth and development in the medium to long-term. About KHAN-I KHAN Technology Transfer Fund I GmbH & Co KG (KHAN-I) is an early-stage life sciences venture fund with € 70 million under management. Their mission is to create value through cooperative drug development partnerships with academic innovators in Europe. KHAN-I focuses on first-in-class therapies for attractive markets with a high unmet medical need. The fund is managed by Khanu Management GmbH, an experienced team of professionals with proven track records in early-stage drug development and academic spin-offs as well as pharma licensing and partnering. KHAN-I received an investment from the European Investment Fund (EIF) with the support of InnovFin Equity, and with the financial backing of the European Union under Horizon 2020 Financial Instruments and the European Fund for Strategic Investments (EFSI) under the Investment Plan for Europe. KHAN-I is also supported by Austria Wirtschaftsservice GmbH (AWS with funds provided by the Austrian Federal Ministry for Digital and Economic Affairs and the Austrian Foundation for Research, Technology, and Development), Max Planck Foundation, and Thyssen’sche Handelsgesellschaft mbH. In addition, KHAN-I sustains a preferred partnership with the Max Planck Society (Max-Planck-Gesellschaft e.V.). More information at www.khanu.de. About Holeček Family Foundation We support people with knowledge, experience and vision in areas we consider important for the development of our society. We provide support in the form of an open opportunity to receive funding from the Foundation and we leave it up to the beneficiaries to decide how to best use the support we grant. We would like to inspire others with our actions, because we believe that giving back to society brings value to us all. Our main area of focus is the Czech educational system. Educated people are a prerequisite for the development of any society and there is therefore a close focus on educational projects in the Foundation’s activities. Good health is a prerequisite for a full and active life of each of us. Therefore, the quality of health care, social care and the health care system is also in the Foundation’s focus. Last but not least, the Foundation supports science and research projects as the main drivers of progress and development in any society. The Foundation mainly supports organisations such as universities or hospitals, which already have a strong potential in the fields mentioned above, but their funding does not always allow them to fully develop their potential. In addition to these main areas of focus, the Board of Trustees also looks at specific innovative projects with the potential of bringing new discoveries or practices. More information at www.holecekfoundation.cz .

i&i Bio, the newly estabilished investment fund powered by i&i Prague and EIF It is our pleasure to announce the start of operations of i&i Biotech Fund (i&i Bio ) in September 2021. This is a great success of i&i Prague team and it brings a new quality and a unique opportunity for early-phase deep-tech spin-offs in Czechia and rest of the Europe. i&i Bio focuses on unique technologies in the fields of Medtech, Diagnostics and Drug discovery. i&i Bio manages more than 45 million EUR and the fund is prepared to invest an average of 2 million EUR (more than 50 million CZK) in a specific project, but in exceptional cases the amount may grow to as much as 4.5 million EUR for a single project. The fund will be joined by further investors in near future including CUIP , the daughter company of the Charles University in Prague. Further details and press release could be found here ! About i&i Bio i&i Bio is a Luxembourg-based venture capital firm that invests in innovative European Life Sciences companies focused on drug discoveries, medical devices, diagnostics, and digital health. The Fund was created thanks to the cooperation of the biotech incubator i&i Prague and the European Investment Fund (see below). With over €45M under management, i&i Bio plans to invest in about 20 early-stage companies. i&i Bio is led by an experienced team of professionals with backgrounds in private equity, healthcare and venture capital supporting entrepreneurs on their journey to global success. Thanks to the close cooperation with the fund’s sponsor, the biotech academic incubator i&i Prague, i&i Bio is supporting and advancing transformative Central European technology companies. For more information visit www.inibio.eu . About European Investment Fund i&i Bio is supported by an investment from the EIF, with the support of: lnnovFin Equity, with the financial backing of the European Union under Horizon 2020 Financial Instruments and the European Fund for Strategic Investments (EFSI) set up under the Investment Plan for Europe. The purpose of EFSI is to help support financing and implementing productive investments in the European Union and to ensure increased access to financing; and the Pan-European Guarantee Fund (EGF), implemented by the EIF with the financial support of the Participating Member States. The objective of EGF is to respond to the economic impact of the COVID-19 pandemic by ensuring that companies in the Participating Member States have sufficient short-term liquidity available to weather the crisis and are able to continue their growth and development in the medium to long-term.

IOCB Tech Group Successfully Represents the Czech Republic at the Prestigious Biotech Conference BIO-Europe 2023 Over 5,000 participants from more than 50 countries and a total of over 30,000 official meetings – that was the outcome of this year's edition of the international BIO-Europe conference in Munich. The Czech Republic was represented by the companies IOCB Tech, i&i Prague, and i&i Biotech Fund (i&i Bio), which are entities associated with the IOCB Tech Group under the Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences. The Czech stand at the event was also supported by representatives of the Consulate General of the Czech Republic in Munich. “IOCB Tech Group regularly participates in BIO-Europe. It is a useful opportunity for us to meet with leading representatives of the biotech, medtech, and life sciences world, as well as investors and scientific research clusters. Thanks also go to the organizers, the EBD Group,” said Martin Fusek, director of IOCB Tech. “BIO-Europe represents a great opportunity for us to meet startups at the forefront of Biotech. Specially, in the CEE region we have found great founders who are shaking the field with their innovative technologies. The event also gives us a chance to reach out to startups which might not have been ready for investment when we originally talked to them, but after some time it is fascinating to see their progress,” adds Roberto Fernandez Alvarez, who represented the incubator i&i Prague with other colleagues. “This year's BIO-Europe was also unique due to the inclusion of a new section of panel discussions on so-called translational funds. These are investors who provide fund financing for the translational phase of research and the technology transfer process. Besides our i&i Bio, four other funds from Germany, Austria, and Belgium were presented, which were also established at significant scientific institutions,” says Jaromír Zahrádka, director of i&i Biotech Fund, adding: “It is evident that such funds have their place in the European venture capital market and often become the initial investor in new projects and biotechnological spin-off companies, which then get the chance to appeal to a broader group of less specialized investors.” The IOCB Tech team conducted more than a hundred meetings and negotiations over three days. “We are particularly grateful for the discussion with the EBD Group regarding the potential hosting of the BIO-Europe conference in Prague, and we are pleased that this vision is also supported by significant clusters such as LISAvienna – Life Science Austria Vienna, BioM Biotech Cluster, Biosaxony association, and many others,” added Martin Opatrný, spokesperson for IOCB Tech. “BIO-Europe represents a great opportunity for us to meet startups at the forefront of Biotech. Specially, in the CEE region we have found great founders who are shaking the field with their innovative technologies. The event also gives us a chance to reach out to startups which might not have been ready for investment when we originally talked to them, but after some time it is fascinating to see their progress,” Roberto Fernandez Alvarez i&i Prague Discussions on cooperation also took place, for example, with the company Symeres, a leading research organization focused on the discovery and development of new drugs. Representatives of the Consulate General in Munich also expressed their support for the IOCB Tech Group, whose visit was a significant recognition of the activities of the IOCB Tech Group. “We are glad to be part of this significant event and present, thanks to IOCB Tech, Czech excellence in the field of life sciences and pharmacy, and thus show the world the innovations and potential of Czech science and technology transfer,” said consul Lukáš Opatrný from the Consulate General of the Czech Republic in Munich. BIO-Europe is an annual meeting of visionaries, innovators, and experts in the field of biotechnology and pharmaceutical research from around the world. Over the past 29 years, BIO-Europe has grown not only in size but especially in becoming the flagship of European biotech. Its international reach is a symbolic gateway to the global life science community. About IOCB Tech The Technology Transfer Office IOCB Tech, s.r.o. is a fully owned subsidiary of the Institute of Organic Chemistry and Biochemistry (IOCB). The main responsibilities of IOCB Tech include the identification of commercially interesting projects at IOCB, analysis of their market potential and patentability, protection of intellectual property, support for the development of the projects in the form of project management, search for commercial partners and negotiation of contractual terms for signing license agreements. IOCB Tech has been involved in the process of negotiating and concluding more than a dozen key license agreements with major pharmaceutical partners such as Gilead Sciences, Merck, Novo Nordisk and SHINE Medical Technologies. In 2017, the company established a subsidiary, i&i Prague, which focuses on funding promising early-stage projects at the Institute and other research and academic institutions. For more information visit www.iocbtech.cz . About i&i Prague The i&i Prague Bio-Innovation Center focuses on the transfer of new technologies into practice. The company was established at the Institute of Organic Chemistry and Biochemistry of the CAS and is mainly dedicated to innovations in the field of drug development, diagnostics and medical devices originating from academic institutions. It supports the creation of spin-off companies and the sale of licenses. So far, the company has participated in the creation of or otherwise supported 15 spin-off companies from five countries, has an equity stake in nine of them and has invested over 70 million crowns. In total, the spin-offs in i&i Prague's portfolio have raised more than CZK 1.8 billion from other investors. In addition to direct financial investments, i&i Prague also assists more than 15 research institutions and universities in the Czech Republic and abroad in the commercialization of innovative technologies. In 2021, i&i Prague started up the i&i Bio investment fund. About i&i Biotech Fund (i&i Bio) i&i Bio is a Luxembourg-based venture capital firm that invests in innovative European Life Sciences companies focused on drug discoveries, medical devices, diagnostics, and digital health. The Fund was created thanks to the cooperation of the biotech incubator i&i Prague and the European Investment Fund (see below). With over €47M under management, i&i Bio plans to invest in about 20 early-stage companies. i&i Bio is led by an experienced team of professionals with backgrounds in private equity, healthcare and venture capital supporting entrepreneurs on their journey to global success. Thanks to close cooperation with the fund’s main sponsor, the biotech academic incubator i&i Prague, i&i Bio is supporting and advancing transformative Central European technology companies. For more information visit www.inibio.eu . About EIF i&i Bio is supported by an investment from the EIF, with the support of: lnnovFin Equity, with the financial backing of the European Union under Horizon 2020 Financial Instruments and the European Fund for Strategic Investments (EFSI) set up under the Investment Plan for Europe. The purpose of EFSI is to help support financing and implementing productive investments in the European Union and to ensure increased access to financing; and the Pan-European Guarantee Fund (EGF), implemented by the EIF with the financial support of the Participating Member States. The objective of EGF is to respond to the economic impact of the COVID-19 pandemic by ensuring that companies in the Participating Member States have sufficient short-term liquidity available to weather the crisis and are able to continue their growth and development in the medium to long-term.

Enantis, a Masaryk University spin-off company, concluded a license agreement for the production of stabilised growth factor FGF2. After months of negotiations, we are delighted to announce that Enantis, one of i&i Prague´s first supported start-ups, has entered into a global license agreement with one of the top providers of life science solutions with ther innovative FGF2-STAB® molecule and its use in research and cell therapy market. “Having a licensing deal with a company from Fortune magazine’s yearly list of 500 largest US companies is a significant milestone for us,” says Roman Badik, CEO of Enantis. Under the terms of agreement, Enantis along with Masaryk University have granted worldwide royalty-bearing license to manufacture FGF2-STAB® and develop new products containing this patented molecule. More details including the name of the licensee cannot be disclosed as per the terms of the license. Enantis, Masaryk University’s first biotechnology spin-off company, has recently concluded a license agreement with a leading global distributor of materials for science and research. This will enable to increase the production of FGF2-STAB, a patented fibroblast growth factor, making it available to laboratories all over the world. Revenues from sales will also go to MU. Growth factors are among the most important biomolecules in living cells. The synthesis and degradation of growth factors at the various stages of cell division and differentiation enable living organisms to develop and grow properly. Fibroblast growth factor 2 is among key molecules in cell cultivation media used to grow embryonic stem cells, which have broad applications in biomedical research and clinical practice. Fibroblast growth factors are proteins supporting cell growth and division. Petr Dvořák and Pavel Krejčí from the Department of Biology at the Faculty of Medicine were among the first to work with these factors, focusing on FGF2. They discovered and characterised some of its properties, but more importantly, they managed to stabilise the factor using protein engineering. This means they modified it to remain functional for over twenty days at temperatures of around 37 degrees Celsius, which is critical for biological applications. Leveraging their close co-operation with the teams at Loschmidt Laboratories, RECETOX, and the Department of Experimental Biology at the Faculty of Science, they agreed to test the newly developed FireProt stabilisation platform on these biomedically interesting but unstable molecules. “FireProt is a computational system that enables us to search for suitable modifications of molecules to make them useful in practice. FGF2 was the first system with applications in biomedicine on which we successfully used our platform,” said Jiří Damborský, describing the steps towards the recent success. Modifications of FGF2 through protein engineering took three years and focused on the protein’s thermodynamic stability. “We gradually substituted certain amino acids in its structure for different ones, or in other words we deliberately created point mutations. This process strengthened the interactions between the amino acids, thus improving the overall robustness of the protein. The design of the mutations and their construction was quite fast, but then came a long period where we had to test their influence on the FGF2’s biological activity. We were very careful not to damage the protein, so we always tested only a single variant out of thousands of candidates,” added David Bednář, one of the creators of the FireProt platform. Stabilised FGF2 can be used by scientists in their work with stem cells because it stimulates cell division and can be used in cell therapies, treatment of slow-healing wounds such as burns and in the treatment of diabetes. The molecule is protected by a patent that is jointly owned by MU and Enantis. The product named FGF2-STAB secured a European patent and patent proceedings in other countries are pending. Enantis is responsible for the commercial applications of the molecule. After a year of negotiations, the company managed to conclude a license agreement with a US company which will manufacture and further develop the application possibilities of the growth factor. “The negotiation was not easy, but we’re happy that such an important global company decided to license our technology and satisfy the growing demand for our product,” said Roman Badík, CEO of Enantis. While the name of the global company and other details of the license agreement have not been made public, it represents a breakthrough in the history of Masaryk University. “Each year, MU enters into dozens of licensing agreements for the intellectual property developed at the university. However, in terms of future benefits, this may be one of the most important contracts signed so far,” said Radoslav Trautmann, head of MU’s Technology Transfer Division, adding that it marks a milestone in biotechnology not only for the South Moravian Region, but the Czech Republic as a whole.

New startup in our portfolio: Sophomer's technology will make immunoassays faster, easier and cheaper The originators of SophoMer in their chemical laboratory. Prague, February 7, 2023. The academic startup Sophomer today announced the signing of a licensing agreement to commercialize a technology that can replace the traditionally used bovine serum albumin (BSA) in immunoassays. The newly licensed technology has been developed in collaboration between the Institute of Macromolecular Chemistry of the CAS (IMC), Elisa Development and the bio-innovation center i&i Prague. The signing of the license agreement also officially accelerated the activities of the startup. Sophomer has also joined the portfolio of i&i Prague. BSA is one of the oldest industrially derived proteins. In immunoassays it is primarily used as a blocker, which makes the measurements more accurate by suppressing unwanted signals. It is derived from bovine serum, which poses a number of problems. These include the risk of pathogen transmission, which means that this material must be tested rigorously and expensively and its use is subject to certification to confirm its safety. "Logically, there is no such risk with our technology, as our product is fully synthetic. This eliminates other problems, whether it is the variability in reproduction of production, the relatively high price, which increases with the requirement for higher purity, or the complex disposal of the material. The ethical aspect of the whole matter is also not negligible," says Jan Plicka of Elisa Development and a leading expert in the development of diagnostic devices. Sophomer's technology will find application wherever immunoassays is a basic working tool. It has the potential to greatly assist in biochemical laboratories, in the development of In Vitro Diagnostic (IVD) devices, in the monitoring of environmental contaminations, in food laboratories for the determination of allergen content, or in immunoassays in veterinary laboratories. "Our ambitions continue to grow as our research progresses, but the main idea remains the same: to make the work of our fellow developers and researchers faster, easier and, to a large extent, cheaper. We want them to be able to focus without distraction on their own ideas and the challenges that their demanding, extremely important and at the same time low-visibility work brings to the public," adds Martin Burkhard, CEO of Sophomer. A great example of technology transfer The creation of the start-up Sophomer is a prime example of technology transfer and the linking of science and industry. "At the beginning of the story there was a need for diagnostic test manufacturers, which we were able to identify thanks to our network of contacts. We then searched academia for a technology suitable to address this need. We found a suitable technology at the Institute of Macromolecular Chemistry and, after successful validation tests, we assembled a joint development team," says Jiří Moos from the i&i Prague Bio-Innovation Center, which has been behind the project from the very beginning. Among other things, i&i Prague also participated in the creation of a startup that has been included in the portfolio of this bio-innovation center. "We are currently helping to find customers for the developed polymer Sophomer F10, while continuing to co-develop other products. We are also well on our way to arranging the first investment that will help kick-start the company's activities," adds Jiří Moos. Synthesis of a polymer product at a defined temperature in a bath. A team of scientists at the Institute of Macromolecular Chemistry began developing the technology that led to the establishment of Sophomer about two years ago. "I am very pleased that we have managed to bring our joint efforts to the successful signing of a licensing agreement. We have thus taken an important step towards ensuring that the quality work of our scientists finds its application in real life. This builds on our previous successful transfers of basic research results into practice. I believe that this technology from the Institute of Macromolecular Chemistry will significantly simplify the performance of immunoassays," adds Jiří Kotek, Director of the Institute of Macromolecular Chemistry of the CAS. Contact Martin Kovalčík, 777 472 863, kovalcik@iniprague.com The signing of a licensing agreement from the right: Jiří Moos, Martin Burkhard, Jiří Kotek, Jan Plicka and Alexandr Krajhanzl The signing of a licensing agreement Jiří Kotek, Director of the Institute of Macromolecular Chemistry of the CAS The signing of a licensing agreement Alexandr Krajhanzl, i&i Prague The signing of a licensing agreement from the right: Jiří Moos, Martin Burkhard, Jiří Kotek, Jan Plicka and Alexandr Krajhanzl 1/6 About Sophomer Sophomer Ltd. is an academic startup founded by a group of people who come from both industry and academia. Their vision is to bring together two seemingly unrelated fields: immunoassays and polymer chemistry to help anyone who uses immunochemical assays in their work. One of the startup's first and widely applicable products is SophoMer(TM), a polymer that can replace the widely used bovine serum albumin in immunochemical assays, currently a component without which the development of immunological analytics is seemingly unimaginable. Read more here: www.sophomer.com . About Institute of Macromolecular Chemistry of the CAS, v. v. i. (IMC) The Institute of Macromolecular Chemistry of the CAS, v. v. i. (IMC) is the largest research institution in the field of polymer and polymer materials research in the Czech Republic. In this field, it is also among the most important academic research centres in the world. IMC has a knowledge potential of more than 120 scientists in the key fields of macromolecular chemistry, physical chemistry and polymer physics, as well as in related fields such as biochemistry or biomedical or materials engineering. Although the institute is primarily focused on basic research, it also works closely with the application sphere, as evidenced by a number of applied basic research results. Read more here: www.imc.cas.cz/eng . About i&i Prague The i&i Prague Bio-Innovation Center focuses on the transfer of new technologies into practice. The company was established at the Institute of Organic Chemistry and Biochemistry of the CAS and is mainly dedicated to innovations in the field of drug development, diagnostics and medical devices originating from academic institutions. It supports the creation of spin-off companies and the sale of licenses. So far, the company has participated in the creation of or otherwise supported 15 spin-off companies from five countries, has an equity stake in nine of them and has invested over 70 million crowns. In total, the spin-offs in i&i Prague's portfolio have raised more than CZK 1.8 billion from other investors. In addition to direct financial investments, i&i Prague also assists more than 15 research institutions and universities in the Czech Republic and abroad in the commercialization of innovative technologies. In 2021, i&i Prague started up the i&i Bio investment fund. About Elisa Development Elisa Development, in cooperation with the Food Research Institute Prague, has previously developed and marketed several ELISA format immunoassay kits for the quantitative detection of food allergens in food and food raw materials. During this development, the company's staff has repeatedly faced the problem of variable quality of BSA, which is very often used as a blocker of non-specific interactions. These problems were the impetus for the idea of replacing BSA derived from bovine serum with a synthetic polymer, which led to the approach to i&i Prague and subsequently to the collaboration with the Institute of Macromolecular Chemistry.

Dracen Pharmaceuticals Announces the Initiation of First-in-human Study of DRP-104 in Adult Patients With Advanced Solid Tumors! Dracen Pharmaceuticals, Inc. has announced the start of a clinical trial of DRP-104, an anti-cancer substance developed by the team of Pavel Majer at IOCB Prague in collaboration with Johns Hopkins University in Baltimore (USA) @johnshopkinsu . The first patients have received the substance, and the initial phase of the trial will last approximately three years. “The initiation of the phase one clinical trial of our substance is excellent news and an important milestone on the path towards a new treatment for oncology patients,” said Pavel Majer, Head of the drug discovery group at IOCB Prague. Learn more on IOCB page or Dracen page !

Green chemistry start-up Sulfotools closes seed round with i&i Prague. Darmstadt (Germany) 21.04.2021 – Peptides, small proteins with a broad range of biological properties, are used as active ingredients in many different products, e.g. as therapeutics (cancer treatment), in cosmetics (anti-aging products) or nutrition products. However, only few people are aware that the chemical production of such compounds is associated with immense consumption of toxic solvents and CO2 emission. Sulfotools GmbH, as spin-off of Technical University of Darmstadt, has developed a sustainable chemical peptide production method using water instead of toxic solvents, the Clean Peptide Technology (CPT). Together with the Czech biotech incubator & investor i&i Prague, Sulfotools will commercialize its technology to make peptide production more sustainable and eco-friendlier. For economic reasons, the majority of marketed peptides are produced chemically via the socalled solid-phase peptide synthesis (SPPS, state of the art). Each year the peptide industry consumes tens of thousands tons of environmentally harmful, hazardous and expensive organic solvents for the manufacturing. Additionally, the application of such solvents is associated with significant risks for humans and the environment (immense CO2 emission). N,N-dimethylformamide (DMF), the most common solvent used for peptide synthesis is toxic, teratogen and classified as Substance of Very High Concern (SVHC) by the European Chemicals Directive REACh. Especially, in cosmetic peptides residues of these toxic solvents are problematic and therefore, the purification of such peptides is very cost intensive. Sulfotools Clean Peptide Technology (CPT) combines the advantages of chemical synthesis with the mild and sustainable conditions of recombinant production. It is based on novel watersoluble building blocks, the use of which allows for the complete substitution of toxic organic solvents with water during the manufacturing process. Additionally, CPT includes an efficient purification system, a simple wastewater treatment option and due to intrinsic fluorescent properties, for the first time a real-time monitoring of the reaction progress, altogether leading to cost savings of up to 50% and at the same time to an eco-friendly production. Moreover, solvent-free end products can be produced which is an immense advantage for the application in the field of cosmetics and food supplements. Especially cosmetic and nutrition companies using CPT can advertise the benefits of a sustainable production directly to their endcustomers. The Sulfotools GmbH was founded in March 2016 by an interdisciplinary team from Darmstadt, Germany, which is driven by a clear vision: The greening of chemical peptide synthesis. The idea was born during the PhD research of Sascha Knauer at the Technical University of Darmstadt and filed in as patent application. Within the Exist I and II program of the German Federal Ministry of Economics and Energy (BMWi) the Clean Peptide Technology was developed to market-readiness. This included the demonstration of the applicability of the CPT by the successful peptide syntheses used in cosmetics or pharmaceuticals as well as in feasibility studies for renowned industrial customers. Moreover, the manufacturing processes for the water-soluble building blocks were optimized and are ready for up-scale. Together with i&i Prague Sulfotools will scale-up of the manufacturing of the water-soluble building blocks and start the commercialization of the technology. (www.sulfotools.com )